ULTRAPrep® BAC Genomic DNA Bacteria Kit

Total nucleic acid and DNA purification from gram-positive and gram-negative bacteria

The ULTRAPrep® Genomic DNA Kits for bateria provide an easy, safe and reliable method for the isolation of pure genomic DNA.
With this kit it is possible to isolate total nucleic acids or, after RNase- treatment, DNA within a short time (30 min.). The cells are treated with lysozyme (15 min.) and are afterwards lysed in a highly chaotropic milieu. This prevents the degradation of the nucleic acids by cellular nucleases. The prurification protocol itself only requires a few steps. The nucleic acids respectively the DNA are eluted high molecular (>50 kb) and high concentrated in a small volume.

Qick processing by high yields up to 80 ug DNA, protocol for difficult to lyse bacteria

Yield (1-1,5 ml culture):
Halobacterium: OD 260/280 = 1,83 Yield (ug) = 3,7
Pseudomonas: OD 260/280 = 1,92 Yield (ug) = 16,0
Synechococcus: OD 260/280 = 1,80 Yield (ug) = 4,5
E. Coli (5 ml): OD 260/280 = 2,00 Yield (ug) = 79,5
Bacillus: OD 260/280 = 1,80 Yield (ug) = 5,1

Declaration of Conformity:
As in vitro diagnostic medical device classified as follows according to the directive on in vitro diagnostic medical devices 98/79 EC and marked with the CE-IVD sign.

Further specification, ordering or product information you will find if you open or download the new AHN Catalogue on this home page !