ULTRAPrep® Total-RNA BAC Kit

Total-RNA isolation from Gram-negative and Gram-positive bacteria !

The ULTRAPrep® Total-RNA BAC Kits for bacteria provide an easy, safe and reliable method for the isolation of exceptional RNA quality.

The kit contains all buffers and reagents required for isolating total RNA from gram-psotive and gram-negative bacteria, including high activity RNase free DNase I. It presents remarkable features of timesaving, easy handling, speed and low cost for purification of stable total RNA from gram-positive and gram-negative bacteria. Esay procedure: after harvest,cells are submitted to a short muralytic treatment (e.g. E.Coli 15 min.), treated with lysozyme solution or the AHN BAC-lysis reagent (for gram-positive or gram-negative bacteria) and then lysed in a highly-chaotropic buffer (guanidine hydro-chloride). This buffer inactivates RNase immediately and completely. Subsequently, the RNA is purified in only a few steps on the special AHN SAM-filter-column, in which DNA digestion by RNase I occurs. The RNA is finally eluted at high concentration with the RNase free elution buffer. Preparations from up to 5 x 10 (high 9) cells.

Quick procedure (30 min.) with high yield up to 80 ug total RNA, suitable for gram-positive and gram-negative bacteria, special lysis agent for hard-to-lyse gram-positive or gram-negative bacteria optional, exceptional RNA quality for all downstream applications, including RT-PCR, qRT-PCR and Northern Blotting !

Declaration of Conformity:
As in vitro diagnostic medical device classified as follows according to the directive on in vitro diagnostic medical devices 98/79 EC and marked with the CE-IVD sign.

Further specification, ordering or product information you will find if you open or download the new AHN Catalogue on this home page!