ULTRAPrep® Total-RNA Funghi Kit

Total-RNA isolation from yeasts and funghi !

The ULTRAPrep® Total-RNA Funghi Kits for yeasts and funghi provide a cheap, easy, safe and reliable method for the isolation of exceptional RNA quality.

Extracting high purity RNA rom yeasts and funghi by simple procedure. Two protocols are offered for efficient lysis of yeast cells: one protocol uses enzymatic hydrolysis (lyticase) of cell walls, the other mechanical treatment by grinding under liquid nitrogen of cells resistant to lyticase. Lysis is achieved with a high-chaotropic buffer (guanidinium hydrochloride), without use of mercaptoethanol. After addition of binding buffer and binding of the nucleic acid to the filter-matrix, the DNA is degraded on the column by supplied of higly active RNase-free DNase I. The isolation of the total RNA takes only 10-15 more minutes.

Two protocols; enzymatic cell wall hydrolysis (lyticase) and mechanical disintegration (grinding). Stable RNA preps within only 60-85 minutes. Cost saving because RNase-free DNase I is included!

Declaration of Conformity:
As in vitro diagnostic medical device classified as follows according to the directive on in vitro diagnostic medical devices 98/79 EC and marked with the CE-IVD sign.

Further specification, ordering or product information you will find if you open or download the new AHN Catalogue on this home page!